Miniscope V4 connection problem

Rob Ellingford

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Nov 25, 2020, 8:31:02 AM11/25/20

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Hi

I have just tried connecting our newly assembled V4 miniscope to a computer for the first time and it seems like the scope is failing to connect to the DAQ software. I recurrently receive the error message "Miniscope retrieve frame failed" (please see below image). The 3 green LEDs on the DAQ and red LED on the miniscope are lit and it is detected as a device on the computer. I also believe I have the correct deviceID number in the User Config file and the latest version of the software (Open GL = 4.4). Is there any process I may have forgotten to do before connecting the scope?

Many Thanks

Rob

Miniscope issue.png

Eric Melonakos

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Nov 25, 2020, 10:26:00 AM11/25/20

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Hi Rob,

This may be a long shot, but did you recently get your DAQ from Open Ephys? They recently sent out an email about a mistake they made that's solved by opening the box and moving the jumper from K1 to K2. I was having a similar issue to you and that fixed it.

Best of luck,

Eric

Daniel Aharoni

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Nov 30, 2020, 2:47:22 PM11/30/20

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Hi Rob, has this connection issue been resolve? If not please let me know and we can walk you through some other possible solutions.

Rob Ellingford

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Dec 2, 2020, 12:06:57 PM12/2/20

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Hi Both

Sorry for the delay in replying. Our DAQ has a jumper across K2 so I don't think it is the same issue you had Eric but thank you for the suggestion. Daniel, I am attempting to follow the instructions on the GitHub firmware page to program the DAQ box (https://github.com/Aharoni-Lab/Miniscope-DAQ-Cypress-firmware). Is this a mandatory process and could the fact that I am yet to do it the likely reason that the Miniscope is failing to connect? Any other suggestions you have would also be welcome

Best wishes

Rob

Rob Ellingford

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Dec 2, 2020, 12:16:03 PM12/2/20

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PS. If it is necessary to programme the DAQ box would you be able to provide some more detailed instructions for step 1 on the linked github instructions as I am unfamiliar with the processes. My understanding is that to programme the DAQ it needs to be booted in EEPROM mde which is achieved by using a jumper on both K2 and K1? If so do we need to supply our own jumper for the additional connection?

William Mau

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Dec 2, 2020, 12:16:08 PM12/2/20

to Rob Ellingford, Miniscope

Yes it is mandatory. And it worked instantly for me after I updated the firmware.

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Richardson Leão

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Dec 2, 2020, 12:35:23 PM12/2/20

to William Mau, Rob Ellingford, Miniscope

Have u tried a different computer? Usb3 is finicky. In our lab (Brazilian coast, 98% humidity), we have only a couple of laptops that have one or 2 ports that give no red screen.

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Ian Jones

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Jan 6, 2021, 1:48:32 PM1/6/21

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Hello,

Did you ever find a fix for this problem? I'm experiencing the same thing.

Thanks,

Ian

Rob Ellingford

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Jan 7, 2021, 6:25:17 AM1/7/21

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Hi Ian

Yes I did, the issue was that I needed to update the Firmware on the DAQ. Try following the tutorial steps in the this link (https://github.com/Aharoni-Lab/Miniscope-DAQ-Cypress-firmware/wiki/Uploading-Firmware-to-DAQ). I would add the following details that aren't specified in the tutorial and took me a little while to figure out.

1) You will need to purchase a shorting link to set up the DAQ for a new firmware upload (https://uk.rs-online.com/web/p/jumpers-shunts/2518682/)

2) Cypress USB Control Center is opened within the EZ USB suite (click the icon of a tree in a blue circle on the toolbar of EZ USB suite)

3) If you don't see Cypress USB Bootloader in control center, check in your device manager whether a device called "Westbridge" is listed under other devices. If so, follow this tutorial (https://community.cypress.com/t5/Knowledge-Base-Articles/Trouble-Shooting-Guide-for-the-FX3-FX3S-CX3-Enumeration/ta-p/248415), after which Cypress USB bootloader should appear in control center. You can then follow the rest of the firmware upload tutorial