Hi Federico,
thanks for your quick reply! It worked. Good to be re-assured that it wont have any effects on the data analysis.
I am trying to find a reason why I cant find responsive cells in my new v4 miniscopes recordings. I would be glad if you, Daniel or anyone who feels confident enough could share her or his thoughts on the following:
Just by looking at the live image (raw or dF/F) during the on-going recordings I can clearly see that whenever the animal receives the reward in a trial, almost all imaged cells go silent from the timepoint of reward retrieval until a few seconds after. However, I am not able to see that when creating PETHs aligned to the reward event. The peaks for each trial are widly distributed leading to an average activity trace that is flat and near to zero for all cells (see left figure for example cell with traces for each trial).
On the other hand, with my older recordings using the v3 miniscope (and the older DAQ box and software) I can clearly see that cells increase or decrease their activity near the timepoint of the event (see middle and right figure). Also, the traces are simliarly aligned for each trial and the amplitude is much higher.
All traces were pre-processed using MIN1PIPE and what is plotted above are the output traces without any normalization. Importantly, the traces of the left figure were pre-processed with the latest version, where as the traces of the middle and right figure were pre-processed with an older version from 2019. Im about to find out if that might be the reason (but I doubt it).
If anyone could help me with this, I would be very grateful.
Best,
Martin