Voltage sensors compatible with Miniscope V4

[Isabel Crisostomo]

Good day everyone,

My lab is currently setting up Miniscope V4, and we are trying to check for voltage sensors that are compatible with the set up. There's not a lot of literature on Miniscope V4 yet (so far, most of the papers I've seen use V3) so I was wondering if I can ask for recommendations here. 

Thank you! 

[Daniel Aharoni]

Hi,

Currently we do not know of any Genetically Encoded Voltage Indicators (GEVIs) that are compatible with standard epifluorescent miniature microscopes including the V3 and V4 Miniscope. The main reason for this is the current generation of GEVIs are just too fast and dim for the types of image sensors that are using in head-mountable miniature microscopes. While the V4 Miniscope can run at up to 480FPS, there is just no way it can collect enough light from GEVIs to have a measurable signal above noise. That being said, there are some groups developing modified Miniscope for voltage sensors that are making progress in this field. Caleb Kemere's group would be a good place to start looking into this: https://www.biorxiv.org/content/10.1101/2020.11.03.367466v1.full.

[cke...@gmail.com]

Hi!

As Daniel says, we've been working on a Miniscope that might work for voltage sensing. In addition to dimness, some GEVIs are also prone to bleaching. I hope to have some more data on this topic in a few months, so feel free to just keep any eye for an updated version of that Biorxiv paper or send me an email!

Caleb

[Isabel Crisostomo]

Thank you Daniel and Caleb for your responses. They're helpful to us. Will keep an eye on the Biorxiv paper and reach out if we have any questions!

Isabel