I am using four miniscope version 4 which all shows good focal points and neural ca2+ activity. However, I found out that three miniscope show darker view when observing same neural populations. When I measured the excitation LED using Thorlab power and energy meter console, I found out that four miniscopes have different LED intensity as following:
1st : 0.99mW
Indeed the one with lower LED intensity showed darker view. I wonder whether this is the issue of LED itself (so, if i change the LED, would it change the led intensity?), or something inside the PCB board. Thanks!