Despite many controls such as double-checking the same mice in the 2-photon and imaging constant expression of microglia in CX3CR1 mice, I’m having issues imaging neuronal activity through cranial windows in Thy1GCaMP6s mice with the V4 Miniscopes.
I tried many variations of cranial windows, coverglass depths, and cortical areas.
My goal is to image in the least invasive way possible, so I would be rather not implant a relay lens.
I suspect that I am still not reaching a sufficient depth to image L2/L3. Has someone resolved a similar issue?
Thank you for any advice/suggestions!