Experience with GCaMP8m or GCaMP7f

[Alfredo Elhazaz]

Hello all,

We have been using the v3 miniscopes with GCaMP6s for a while in our lab with good results, but would like to upgrade soon to a newer sensors. We image mouse orbitofrontal cortex (deep, we use a 0.6mm relay lens) at 15 Hz.

Specially interested of people's experience with GCaMP8m, since i've read some reports of signal being too bright and adding a lot of background fluorescence. Has anyone found a virus/titer that works for them?

We're also interested in 7f, for those that have successfully used it, which construct and at which dilution did you use it?

Thanks!

Alfredo