Dear all,
we were running experiments with the V4 miniscopes and started to analyze them using minian. We are quite happy with the preprocessing steps and it's also cool how the pipeline leads one through the parameter exploration / selection.
However, we see largely prolonged decay times in the computed calcium traces C, if compared to the raw traces. I know there is no single parameter to drive for this, but I have played around with the others quite extensivley and couldnt find settings that have a major impact on the decay times. Caiman has a parameter to play with for decay times, and without tweaking any of the native settings, the deconvolved traces look much better on the same data set already (the fov seems to be reversed as well).
Did anybody encounter similar phenomenons on decay times and/or has tips how to deal with it? For example, are there parameters in the source code one can change to tweak that?