Hi,
I'm working on a dataset consisting of 12 microsatellites for 6 populations. The species is a pond-breeding amphibian, and the ponds are relatively new (created during the 1900s). So, there are certainly several incidences of population splitting through founding events.
I am mostly interested in migration rates, but I understand that population splitting must be accounted for. I have read some of the comments in the group and I see that using "D" (divergence with migration) is not recommended with high migration rate because divergence time will be biased. I have reason to belive that migration have been relatively high after divergence. My question is : are migration rates also biased when using "D"?
It is not a huge problem if divergence times is not accurate. In fact, since I have the year of the creation of each pond I have an idea of that already. But the migration rates I am very concerned about.
Many thanks!
Hanne