Calculating immigrant number between four populations

[Jesus Antonio Rocamontes Morales]

Hello Migrate support group,

I am currently trying to infer the direction of gene flow in four populations and using migrate-n to do so. I am using mtDNA sequences and was hoping you could give me advice how to improve my analysis and check if there are issues with my parmfile. Currently using a Bayesian approach and so far the posterior distribution of M looks decent for all routes except for one, but Ne still seems to be uncertain. Also using 5 replicates and heating {1.0, 1.2, 3.0,1000000.0} to improve my runs.  I attach here my parmfile and one my runs.

Thanks for your help.

Best regards,

Tony

[Peter Beerli]

I gather that this is not very variable data, but your prior for theta is too narrow try, a range of 0.0 to 0.005

if that still bounces on the max prior then perhaps try 0.01 (and increase the number of bins to 5000)

Peter

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