FM 4-64 membrane stain

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bacillusjunkie

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May 17, 2011, 4:12:42 PM5/17/11
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Hi,

Can I use FM 4-64 membrane stain images to define cell outlines
instead of phase contrast images?

emilyh...@gmail.com

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Feb 28, 2013, 9:34:33 AM2/28/13
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Hi,

I have tried to use fluorescence images of FM 4-64 stained cells instead of phase contrast, but so far without luck. It is probably possible to convert the image so that it looks more like a phase contrast image somehow. Anyone here that has any experience with that? It would be very helpful with some advice.

Jacobs-Wagner Group MicrobeTracker

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Jun 24, 2015, 12:54:16 PM6/24/15
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Try to set the invertimage parameter to 1 and that should do it.
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