Hello everyone,
I am using MicrobeJ to detect fluorescent foci that are moving in bacterial cells, and I want to study if some locations in the cell contain more foci.
Especially, I would like to measure the distance of each maxima to the parent cell poles and center. For the center, I just recorded the feature PARENT.distance.midcell and the result seems coherent, but for the distance to poles I am a bit lost.
In the results table, there are 3 different features: PARENT.distance.pole, PARENT.distance.pole1 and PARENT.distance.pole2. What is the difference between them ? In particular, the PARENT.distance.pole seems to take alternatively the value of PARENT.distance.pole1 and PARENT.distance.pole2, and the occurence of each seems random (not 50%-50%). Do I miss something in the definition of the poles ?
Thanks in advance,
Marie