How can I analyze a time lapse?

[afroz...@gmail.com]

I have been able to segment cells in a frame. But I would like to segment multiple frames of the time lapse while i maintain the same ID of the cell.

[MicrobeJ]

Hi,

To track the cells you must use the tracking option (the small clock). Once tracked the cells are organized under Trajectories with a common ID.

Let me know if you have any question,

Ad

[Eric Young]

Hello, to follow up on a similar question: 

I would like to maintain the ID/dimensions of the same cells across a time-lapse. It seems like the beginning frame the cells can be identified, but as time goes on, the distribution of fluorescence changes. This then confuses cell boundaries in the bacterial detection. I do not have another channel for reference unfortunately.  

Would there be any way I can preserve the coordinates from the beginning frame and copy them to the following frame(s) for further analysis?

Frame 1:  

Frame 14: 

[Eric Young]

Sample images

[Andrés Flórez]

I have the same problem, I still haven't figure out how to keep cells with the same ID

[nik...@gmail.com]

Hi MicrobeJ et al.,

Unfortunately, I have the same issue. Is there a solution for this question?

Best regards,

Terrens