Caulobacter crescentus stalk detection

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PG90326

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Feb 7, 2024, 5:40:35 AM2/7/24
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Hi MicrobeJ community, 
I am trying to analyse some images of caulobacter crescentus with fluorescence in the polar stalk of caulobacter. Unfortunately I don't acheive to detect the stalk with the associated cell body. 
Is there microbeJ sets up that could allow me to analyse the stalks ? If not, do you have other recommandations (may be another software?) for that kind of polar stalk analysis ? 
Thank you
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