Nucleoid area measurement

[uw.madison.e...@gmail.com]

Hi,

I am so excited about this plugin but am having a lot of issues figuring out the proper way to measure nucleoid compaction (area/width/length) from DAPI staining as compared to a membrane stain for the rest of the cell length/width. I saw that there is a nucleus function but I cannot figure out where it is in the plugin. Is there a tutorial for the nucleus function?

Should I measure the DAPI stain as a maxima?

I am not familiar with microscopy and using imageJ/MicrobeJ normally so this is a huge learning curve for me!

Any help would be appreciated. 

[Mary Salliss]

Did you manage to fix this problem? I'm also looking to do this and I cant figure it out?

Thanks! :)