Hi all,
I want to calculate the Good's Coverage Estimator using my data from MG-RAST shotgun sequenced samples.
But to calculate Good's I need:
1 - (F1 / N)
where F1 is the number of singleton OTUs and N is the total number of reads (total number of species sampled or total number of reads count?).
Any clue on how to get singleton's number per sample?
Thanks,
Jose