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William Patton
Dec 2, 2019, 4:07:44 PM12/2/19
to MG-RAST
Hello all,
I have 8 2x150bp FASTQ files sequenced on Illumina HiSeq. They are data from the sequencing of a lichen, which contains eukaryotic fungi as well as an assortment of prokaryotes. To avoid misclassification of eukaryotic fungi as a prokaryote, I've been advised to screen this DNA out of the FASTQ files using a reference genome.
I have 8 2x150bp FASTQ files sequenced on Illumina HiSeq. They are data from the sequencing of a lichen, which contains eukaryotic fungi as well as an assortment of prokaryotes. To avoid misclassification of eukaryotic fungi as a prokaryote, I've been advised to screen this DNA out of the FASTQ files using a reference genome.
How would I do this?
I am new to this analysis so all help is greatly appreciated!
-Mac
Folker Meyer
Jan 30, 2020, 2:18:58 PM1/30/20
to MG-RAST
in MG-RAST you'd have to send us pointer to a genome sequence for lichen so that we can set up the screening tool for lichen.
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