MirandaKnight is a veterinarian serving in an instructional role and mentoring students interested in careers associated with animal science and veterinary medicine. Dr. Knight is a Visiting Lecturer in the Department of Animal Science who teaches courses in anatomy and physiology, animal health and disease, reproductive physiology, and companion animal science. Dr. Knight incorporates clinical cases in the courses she instructs to highlight the significance of the anatomy, physiology, and disease processes presented.
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Humans and animals host diverse communities of microorganisms important to their physiology and health. Despite extensive sequencing-based characterization of host-associated microbiomes, there remains a dramatic lack of understanding of microbial functions. Stable-isotope probing (SIP) is a powerful strategy to elucidate the ecophysiology of microorganisms in complex host-associated microbiotas. Here, we suggest that SIP methodologies should be more frequently exploited as part of a holistic functional microbiomics approach. We provide examples of how SIP has been used to study host-associated microbes in vivo and ex vivo. We highlight recent developments in SIP technologies and discuss future directions that will facilitate deeper insights into the function of human and animal microbiomes.
The McAllister Hall addition creates a modern Animal Science building at Berry College, one that promotes student/faculty research interactions and stimulates cross-disciplinary collaboration. With animal science being the largest major at Berry, the 26,500-square-foot addition enhances teaching and research in animal health and production, including genetics, microbiology, and physiology.
Large windows bring an abundance of light into classrooms and labs, putting animal sciences on display. The addition creates a new courtyard space between the original building to promote gathering and events. A new porch offers spectacular views and edge to an active campus path.
The first of many Brasfield & Gorrie projects on the beautiful campus of Berry College, this facility enhances teaching and research opportunities in animal health and production, from behavior and genetics to microbiology and physiology. The expansion added five lab spaces, two teaching labs, and an auditorium-style lecture hall with 14 offices to provide hands-on learning and exceptional instruction for students with a passion for animal care.
Dr. Kim A. Sprayberry grew up in a farm community in the San Joaquin Valley, where her family raised raisin grapes. She attended UC Davis for both an undergraduate degree in Physiology and for veterinary school, from which she graduated in 1988.
Dr. Sprayberry worked in a mixed small-animal and dairy practice in Lemoore for one year and then on the southern California racetracks for several years before returning to Davis for an internal medicine residency in 1994. She completed the residency and became Board-certified in Large-Animal Internal Medicine in 1998, after which she joined the Internal Medicine division of the 50-veterinarian staff at Hagyard-Davidson-McGee (now Hagyard Equine Medical Institute) in Lexington, KY, where she spent 10 years. During the course of this position she also became Board-certified in large animal emergency and critical care, and presently holds Diplomate status in both the American College of Veterinary Internal Medicine and the American College of Veterinary Emergency and Critical Care.
The trial was carried out at the Research Laboratory of the Department of Poultry Science, University of Warmia and Mazury in Olsztyn (Poland) in cooperation with the University of Torino and the Institute of Animal Reproduction and Food Research of PAS in Olsztyn. The experimental protocol was approved by the Local Animal Care and Use Committee (Decision No. 2/2018; Olsztyn, Poland), and the study was carried out in accordance with EU Directive 2010/63/EU for animal experiments. The temperature and lighting program were consistent with the recommendations of Aviagen Group [17]. The birds had free access to feed and water. A total of 480 Ross 308 male broilers at one-day of age were randomly allotted to 8 dietary treatments, each consisting of 6 pens as replicates with 10 birds per pen. The one-day-old chickens were purchased from a commercial hatchery (Animex Group, Sokolka, Poland).
For microbiota analysis, paired-end reads were first merged using FLASH software [30] with default parameters. Joint reads were further quality filtered (at Phred The above-mentioned differences in the polyphenolic compounds in fruit pomaces also influenced the analysed content of total polyphenols and procyanidins in the experimental diets fed to broilers during the starter and grower-finisher feeding periods [see Additional file 2]. In comparison with the control diet, as observed for apple pomaces, the lowest increase in polyphenol levels was noted in AL and AH diets.
Dietary fruit pomace inclusion did not affect the morphometric indices of the broiler chickens. It is well known that the physiological gut development is characterized by long villi and shallow crypts: longer villi are associated with increased absorption of nutrient [24], while shallower crypts reflect a prolonged survival of villi without the need of renewal [39]. On the contrary, lower villus height and greater crypts depth are associated with poor digestion, less absorption of nutrient and poor growth performances [25]. Since both the intestinal morphology and the growth performance of the fruit pomace fed broilers in the current research were unaffected, it is reasonable to hypothesize that fruit pomace meal utilization does not negatively influence gut development or nutrient absorption. Apart from dietary treatments, morphometric indices showed a proximodistal decreasing gradient from duodenum to ileum. This finding is in accordance with the available literature [32, 40] and with the physiological development of the absorption processes. Indeed, the duodenum is the intestinal segment with the fastest cell renewal and the first gut segment to receive the physical, chemical and hormonal stimuli caused by the presence of the diet in the lumen [41]. Furthermore, the jejunum is an important site for nutrient digestion [42].
It is well known that the dietary content and physicochemical properties of different fibre fractions may provoke physiological changes in the small intestine, and subsequently in the lower gut [27, 43]. More soluble fibre as dietary ingredient may considerably affect intestinal viscosity, transit time, digesta moisture, and other indices of intestinal function [44]. In the present study, dietary fruit pomace inclusion significantly increased the SI relative mass when compared to C diets while Ce weight seems to increase in A diet when compared to the other dietary treatments. Addition of fiber to diets largely results in enlargement of digestive tissues, presumably due to increase retention time and digestion of the diet. In fact, Savory and Gentle [45], Kehoe et al. [46], and Williamson et al. [47] found an increase in intestinal relative weight in quails and mallard fed different fiber sources. In the present study, the ileal viscosity was also affected by dietary treatment. In particular, A group showed the highest ileal viscosity. This effect could be partly ascribed to the SDF content and to the different concentrations of non-starch polysaccharides (NSP). As reported in literature, the apple pomace excelled the blackcurrant and strawberry pomaces not only in the content of water-soluble NSPs, but also in the content of NSP monomers such as arabinose, galactose and uronic acid [36]. However, in the present study all the observed viscosity levels were below those that may provoke some undesired physiological effects. Indeed, high digesta viscosity (ranging from 4 to 5 mPas) may not only constrain absorption of nutrients, but also be a stimulus for the overgrowth of microbiota and enhanced putrefactive processes in the lower small intestine [48]. Furthermore, a significant decrease in the activity of α-glucosidase in the small intestine was noticed in A and S animals compared to C and B groups with no variations being detected in the activity of maltase. It has been reported that consumption of high levels of polyphenols may effectively diminish the activity of brush border enzymes in the small intestine [49, 50], thus potentially resulting into less-effective carbohydrate digestion. Indeed, birds fed high-fiber diets had significantly depressed mass-specific small intestinal sucrase activities when compared to birds fed low-fiber diets [51]. Fiber seemed to decrease mass-specific activities of small intestinal sucrase also in geese [51]. This is in contrast with what has previously been demonstrated in chickens fed mannan oligosaccharides, which increased disaccharidase activity [42]. A recent study on growing turkeys fed diets containing 5% of apple, blackcurrant or strawberry pomaces showed significant decrease in sucrase and/or maltase mucosal activity in blackcurrant and strawberry groups, but not in the apple group [36]. These conflicting results could be related to the different fiber components (e.g., pectin vs. cellulose), which can have differential effects on enzyme activities [52]. Also in the ceca, modulation of digestive enzyme activities is one of the mechanism by which the gastrointestinal tract can respond to changes in food composition and quality [51].
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