If I run glam2 on the server with the default settings: glam2 -o set851Out p set851.fasta I get the score of 4612.64. If I am to run the same sequences shuffled 5 times using the Emboss Shuffled (as recommended in the Glam2 manual to improve the score) my score jumps to 19266.8. The score goes up because there are five times the number of sequences so the score should be greater. After reading some papers and the manual, I understand the glam2 output score of each motif represents the addition of individual scores added for each sequence matching to the motif.
My question is how do I interpret the output scores of glam2 results? What score makes a motif significant? Because the larger number of sequences you provide the larger your score gets. In MEME the significance is determined by the E-value. The lower the E-value the more significant the motif correct? A motif with an E-value above .05 is not significant. But how so for glam2 results? Thank you for the help or suggestions.