How to use X! Tandem for protein Identification?

[Messer jorke]

Hi everybody,
I'm a new user of Mass++ and I'm still not able to run all desired functions.
Because I don't have a mascot license I try to use the X! tandem identification.
Therefor I downloaded the proteomdatabase 1.0.0.
But when I start the identifyer und try to change the parameters I'm not able to select a database.
The manual doesn't give me an explanation for my problem, perhaps someone overhere can give me one!?

Thanks a lot

[satstnka]

Dear Messer jorke

 Hi. I'm Satoshi Tanaka,
one of Mass++ developers at Shimadzu Corporation.
 Thank you for downloading and using Mass++.

 ProteomeDatabase installer set the environment variable
"MASSPP_DB_HOME". But it might not be applied immediately.
If you shutdown and restart your PC, this problem might be solved.

> I don't have a mascot license

 Matrix Science has public Mascot server and anyone can use it freely
though there are some limitations. (e.g., You cannot post more than
one thousand peak lists in one time.)
It's available on http://www.matrixscience.com/ .
 You can use it on Mass++ without Mascot license.

  1) Select [Tools] > [Options] on menubar.
     Then the Options dialog appears.
  2) Open the page [Settings] > [Search Engine] > [Mascot]
     on the Options dialog.
  3) Set the following url as [Mascot URL].
        http://www.matrixscience.com/
     [User Name] and [Password] is not required.
  4) Click [OK] button on the Options dialog.

I hope this helps you.

regards,

--
Satoshi Tanaka