[maker-devel] No genes predicted by Fgenesh in MAKER

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Chan Kuang Lim

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Mar 2, 2016, 11:38:03 AM3/2/16
to maker...@yandell-lab.org
Dear MAKER developers,

I am using MAKER 2.31.8, with SNAP, AUGUSTUS and Fgenesh. I have tested my sequences, with many different parameters. MAKER output gives genes predicted by SNAP and AUGUSTUS, but no genes predicted by Fgenesh. I do not get any error message. The sequences FINISHED successful. May I know what are the possible mistake I have done?

Thank you.

Regards,
Chan KL


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Daniel Ence

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Mar 2, 2016, 12:13:45 PM3/2/16
to Chan Kuang Lim, maker...@yandell-lab.org
Hi Chan, Fgenesh is a gene predictor that requires users to purchase parameter files from their company: http://www.softberry.com/. If you didn’t give a Fgenesh file, then you won’t get any predictions. 

~Daniel


Daniel Ence
Graduate Student
Eccles Institute of Human Genetics
University of Utah
15 North 2030 East, Room 2100
Salt Lake City, UT 84112-5330

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Carson Holt

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Mar 2, 2016, 12:51:53 PM3/2/16
to Daniel Ence, maker...@yandell-lab.org, Chan Kuang Lim
Also there is the chance that FgenesH has changed formats slightly for their output (it's happened a couple of times before), so if you are already running with a parameter file you purchased that could be the issues. Look at the STDERR report MAKER produces to see if FgenesH even ran and with what command.

—Carson
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