Hello,
Thank you for creating this wonderful software for splicing analysis.
I have one question that troubles me for a while.
For most of the analysis, I got different number of significant changing LSVs in voila tsv and in voila view.
In one experiment voila tsv, I am using default ---threshold 0.2 --probability-threshold 0.95 --non-changing-threshold 0.05. I have got 673 LSVs.
When I use voila with the default threshold ( abs(E(dPSI)) threshold = 0.2, Confidence Threshold 0.95), I only goty 550 LSVs.
I have looked at the 123 missing LSV, in fact all of them have abs(mean_dpsi_per_lsv_junion) > 0.2, probability_change > 0.95, probability_non_changing < 0.05. Somehow they are not being shown in voila view default view on browser. I can make them appear if I reduce the threshold to 0.1 or confidence internal to 0.9 (instead of 0.95).
The same apply to many different samples I processed. Voila VIEW always got different number of LSV than the TSV under the same threshold.
Why is this the case? Am I missing anything here?
Thank you
Harvey