Thanks San. Median makes sense and FWIW I do think an *option* for sum would be useful, though I agree with your team that median is more useful. Indeed as you point out there are other tracks to display, but for simplicity, I only elected to show the 3 tracks from WT (combined, rep1 & rep2 [here titled 3 & 4, respectively]) and not the 3 tracks from DUB (combined, rep1, rep2).
However, using median does not seem to reconcile what I'm seeing in my splicegraphs.
In case the resolution isn't good enough from the bottom image, I'll tell you the splice numbers. Combined, 5'internalSS (3), 5'terminalSS (11); rep1, 5'internalSS (0), 5'terminalSS (2); rep2, 5'internalSS (3), 5'terminalSS (0). Firstly, this to me shows that the variance is so great between the 2 experimental replicates that this should not have been called on dPSI. Secondly, the combined values are certainly not the median of these two experimental replicates. Am I missing something?
Cheers,
Steve