Error while running Majiq-spel software

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Harisankar Harikumar Sheela

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Jun 11, 2025, 12:00:49 PMJun 11
to majiq...@googlegroups.com
Hi,

Hope this email finds you well. My name is Hari, and I am a graduate student from the University of Michigan. I am currently trying to use the Majiq software for my research. I ran Majiq-delta psi and voila. However when I am uploading an LSV on the Majiq-speI, it shows an error message (please see image attached) and I am not able to see the primers for the LSV. Could you please advise as to how this can be rectified?

Thank you very much,
Hari

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Harisankar Harikumar Sheela
PhD candidate | Department of Molecular, Cellular, and Developmental Biology (MCDB)
University of Michigan
majiq-spel.png

San Jewell

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Jun 11, 2025, 4:16:35 PMJun 11
to Biociphers
Hi Hari,

The "genome" field in the config file is the name of the assembly such as mm10 or hg38. (https://biociphers.bitbucket.io/majiq-docs/getting-started-guide/builder.html) It looks like when you did the majiq build you set the value of genome to a local path to a fasta file instead. (majiq does not need the fasta). You can correct this and re run the build, or, if you only have a few links to copy you can replace that path in the "copy lsv text" to hg38 or whichever assembly instead of the path ("/home/hhsheela/...")

Let me know if it makes sense.

Thanks,
-San
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