[sassie-web/sassie2-feedback][suggestion] 'CMC issue with multiple flexible regions' emre@biochem.uthscsa.edu

[genapp.not...@gmail.com]

subject : CMC issue with multiple flexible regions
from : emre
email : em...@biochem.uthscsa.edu
level : suggestion
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For CMC, I'm getting this message: your low residue plus number contiguous exceeds the number of amino acids-1 (278), reslow = 287 numcont = 6. There are 306 residues in chain A. CMC works fine if I specify only one flexible range (145-151). MMC works fine if i strip the Carb chains BCD and specify both ranges (145-151,287-293).

[mads...@gmail.com]

The last amino acid residue in your PDB is indeed 306, but the first is 28, so you have 306-28 = 278 as shown in the message.  As 141-151 is within range then your flexible region is within the number of amino acids that is checked in the pre-run filter.