Experimental studies of VEGF-C gene for the treatment of chronic obstructive lymphedema in mouse tail model

[lymphedemapeople]

Experimental studies of VEGF-C gene for the treatment of chronic
obstructive lymphedema in mouse tail model

Zhonghua Zheng Xing Wai Ke Za Zhi. 2008 May

Hu XQ, Jiang ZH, Liu NF.
Department of Plastic Surgery, 2nd Affiliated Hospital, College of
Medicine, Zhejiang University, Hangzhou 310009, China.

OBJECTIVE: To study the therapeutic effect of vascular endothelial
growth factor C(VEGF-C) gene for chronic obstructive lymphedema in
mouse tail model which may provide a new treatment for lymphedema.

METHODS: RT-PCR and immunoabsorption were applied to detect VEGF-C
gene expression in fibroblasts and secretion of VEGF-C protein in COS7
cells respectively after pCDNA3.1 (+) VEGF-C transfection. A mouse
tail model of chronic obstructive lymphedema was created. Then the
pcDNA3.1-VEGF-C plasmid was injected into the tail. The effect of
modulating lymphangiogenesis was observed. RESULTS: Compared with
control group, overexpression of VEGF-C enhanced lymphangiogenesis in
vivo and mouse tail skin suffering chronic obstructive lymphedema was
improved by VEGF-C gene significantly.

CONCLUSIONS: VEGF-C can improve the lymphedema through enhancing
lymphangiogenesis.

PMID: 18717357 [PubMed - in process]

http://www.ncbi.nlm.nih.gov/pubmed/18717357?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

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Pat O'Connor
Advocates for Lymphedema
http://health.groups.yahoo.com/group/AdvocatesforLymphedema/

My Life with Lymphedema
http://mylifewithlymphedema.blogspot.com/