Re: Demultiplex reads with multiple primers and barcodes
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Falk Hildebrand
Mar 27, 2018, 9:31:27 AM3/27/18
to LotuS rRNA pipeline
Hey Kristjan,
you need to run LotuS twice, once for each 16S primer. Later the matrices can be combined if you want to (in e.g. R), but I would keep them separate as they likely will have their own biases attached to them.
best,
Falk
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