Import data

[Kombucha UGent]

Dear dr. Hildebrand,

I have some trouble importing my data in the galaxy server and running the lotus2 pipeline (webinterface).

I have 40 samples on which i did 16S rRNA pacbio sequencing (format fastq.gz). I imported 40 folders with the reads of each sample in my galaxy history but when i try to run lotus on the 40 folders it does not work. I have no problem with running lotus on one folder with reads, but when i select the 40 folders it does not work. I imported the data in the tool as multiple datasets and i also made a dataset collection of the folders but both do not work? Would it be possible to give a guideline how to run Lotus on multiple folders with reads ? 

Kind regards,

Stephanie