[Lhs-biohacking] Competent cell protocol

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Lu, Yeping

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Aug 21, 2012, 7:44:54 AM8/21/12

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Here is the competent cell protocol we have been using in our own iGEM lab. Can Tom post your protocol, so we can compare?

Thanks,

Yeping

Competent cell protocol.AG.14.08.12 (1).doc

Tommaso Vannocci

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Aug 21, 2012, 8:17:22 AM8/21/12

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Hi Yeping,

these are my three protocols for competent cells:

I mostly use the first one. It's the fastest and easiest in my opinion but it requires MES (can you check if you have it in the lab?).

See you soon

Tom

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