Hello, I'm trying to use lobSTR but I get an error in the alignment step of BAM files, right after having sorted them with samtools by read name.
I get this warning messages (quite all the shell output is like this):
[lobSTR-4.0.6] 2018-09-04.14:33:12 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12303:15659:13154. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:13 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12303:18562:5111. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:13 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12303:20717:6771. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:13 ProgressMeter: Processed 1570000 pairs 0.19442 passed detection. 0.0104096 passed alignment.
[lobSTR-4.0.6] 2018-09-04.14:33:13 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12303:21509:8207. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:16 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12303:25231:8373. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:16 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12303:25557:6401. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:17 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12304:3243:14521. Is the bam file sorted by read name?
[lobSTR-4.0.6] 2018-09-04.14:33:17 WARNING: Could not find pair for NB501732:8:HLC2VBGX2:1:12304:3403:15207. Is the bam file sorted by read name?
Is there a way to avoid these alignments warning?
Thanks in advance