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Hi Melissa and All I'm having a vcf file of microsatelites generated by lobSTR. My file needs some filteration on some loci. First of all I want to filter acoording to quality because I have some loci with zero quality. My question is, First : Is it true anyway to exclude these kind of loci? Second: I couln't understand how the quality on microsatellite calculated, how much should I care about the quality valuse? Is there any guide which I could refer and clarify me the quality caclulation?? Thank you in advance