value error

[ANIRUDDHA PANDA]

 I am attaching my raw data (both positive mode and negative mode) and also mzXML and mzMl data for your reference. I can not upload all the files in one go, so I am uploading them in multiple steps. Please help us troubleshoot the problem.

Thank you,

With regards,

Aniruddha Panda,

Post Graduate Research Associate,

Yale University, USA.

[Cyrus Papan]

Dear Aniruddha Panda

please note that the .wiff2 files do not contain data, you need to add the .wiff2.scan files they are the larger part of the file pair.  How are you converting the files? are you using msconvert? what are your filters, the hierarchy of filters, if you have more than one? Have you tried opening the files in seeMS?

Best

Cyrus

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[ANIRUDDHA PANDA]

Dear Cyrus,

We are using msconvert without any filter. In msconvert the .wiff2.scan are not visible that means we are not able to convert the .wiff2.scan files. We have checked the .wiff2 converted files in seeMS. They look OK. Could you please use the data I have sent and help us troubleshoot the problem.

Thank you and regards,

Aniruddha. 

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Aniruddha Panda,
RESEARCH SCHOLAR
national centre for biological sciences
tata institute of fundamental research
GKVK, Bellary Road, Bangalore 560 065. India

[Cyrus Papan]

Dear Aniruddha

Hi Anirudda, I can't try the conversion of your data files since you only sent the .wiff2 and not the .wiff.scan-files. So I just tried a conversion of my own IDA file from the X500 and it went ok, both for mzML and mzXML without filters for profile data. The msconvert window and the output in seems are shown below.

You cant see the wiff.scan files in msconvert, but they must be present in the same folder for msconvert to find and convert them.

There is a minor inconvinence: The conversion works in most cases only one file at a time. There will be a new file reader out (hopefully) soon though.

Best wishes

Cyrus

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[ANIRUDDHA PANDA]

Sorry the file size was large. I am sending one by one

On Thu, Sep 5, 2019 at 2:49 PM ANIRUDDHA PANDA <anip...@gmail.com> wrote:

Dear Cyrus,

We kept the .wiif.scan files  in the same folder for msconvert but it did not give us positive results. I am attaching .wiff and .wiff.scan files for your reference. Please help us troubleshoot the problem.

Thank you,

Regards,

Aniruddha.

To view this discussion on the web visit https://groups.google.com/d/msgid/lipidxplorer-discussion-group/11b54415-b69d-7275-e7e2-0dfd91984927%40gmail.com.

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Aniruddha Panda,
RESEARCH SCHOLAR
national centre for biological sciences
tata institute of fundamental research
GKVK, Bellary Road, Bangalore 560 065. India

[ANIRUDDHA PANDA]

[ANIRUDDHA PANDA]

[Cyrus Papan]

Dear Aniruddha,

you have two sample runs in one file. With the direct infusion experiment this is ok from the file size aspect, but msconvert does not seem to like that. Please find the results of the conversion below in seeMS. Conversion worked only for one of the runs but not for the second. Unfortunately, with msconvert you cannot select individual runs from a data file containing multiple runs.

Please acquire each sample run into a separate file, this should resolve the problem.

Keep me posted how it works,

Best

Cyrus

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