LC/MS questions

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Nicholas N

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Feb 26, 2020, 1:55:02 PM2/26/20
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When bootstrapping LipidExplorer to do LC/MS at moderate resolution (about 30000), what do we loose if we split the scan into 5 minute windows? Or small micro windows of one minute or less? 

At what resolution is shotgun unambiguous for most lipid species? 

The data I have is generated in Auto MSMS on an Agilent Qtof around 30k resolution after a c18 HPLC column. 


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