When bootstrapping LipidExplorer to do LC/MS at moderate resolution (about 30000), what do we loose if we split the scan into 5 minute windows? Or small micro windows of one minute or less?
At what resolution is shotgun unambiguous for most lipid species?
The data I have is generated in Auto MSMS on an Agilent Qtof around 30k resolution after a c18 HPLC column.