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CloneEZTM PCR Cloning Kit试剂盒 问题咨询
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xuxiaoke209
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Sep 12, 2009, 12:05:19 AM
9/12/09
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老师,
您好!请问CloneEZTM PCR Cloning Kit试剂盒可以用来构建原核表达载体吗?(会不会发生移码?)
如果可以的话,用pET28a载体构建,在SacI位点酶切进行连接,
PCR正向引物5‘端加的载体序列是?(可以多于15bp吗?)
反向引物5‘端加的载体序列是? (可以多于15bp吗?)
现将pET28a载体序列发给你,烦请举例说明,谢谢!
小徐
广州
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TB074[1].pdf
yangchen307
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Sep 13, 2009, 3:18:47 AM
9/13/09
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您好:
不知您是哪位?咨询我这些问题?
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