LefSe: what happens if no subclass

[John G]

I posted this on the new biobakery help forum, but received no replies so I'm duplicating here before the transition goes final.

Hello,

I’ve seen this question asked a few times on the previous lefse forum but never answered succinctly. If one does not provide a subclass, does LefSe run the wilcoxon? If so, what is it testing and will the result differ from only the KW?

If one does provide a subclass, will the results from the wilcoxon show up in the “.res” file?

Thanks in advance!
John