IF the two items simply carry opposite signs but load on a common factor that represents precisely the same construct, then the negative loadings should have no impact on measurement invariance testing. My concern relates to whether oppositely valenced items do relate to precisely the same construct. There is some literature regarding whether constructs like Satisfaction / Dissatisfaction, Love / Hate, Prosperity / Poverty, etc., are two poles of a continuum or are in fact distinct psychological variables. The latter view suggests that such constructs range only from 0 to +infinity, not from -infinity to
+infinity. One might also suspect a sigmoid function at the extremes of such a construct.
In the past, I have encouraged researchers to use reverse-worded items as attention checks but then to simply discard them, as I suspect the items are generally contaminated. If you want to keep them--whether you reverse-code them or not--you will very likely want to include a residual covariance for these two items. You might actually try including a secondary factor, just to see if the factor could provide some additional predictive ability--which may quite possibly vary by group. However, if the factor does not predict, then the model will fail identification--and the loadings may be quite weak, though strong enough to imply poor fit if this additional covariance is not permitted.
The negative loadings will affect calculation of Cronbach's alpha, and maybe composite reliability, depending on exactly how you calculate that, but for calculation purposes you could just reverse the signs of the loadings manually.