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Hi, I noticed the SHERLOCK assay incorporates RNA extraction (Kellner et al, Nature Protocols). Do you envision RNA extraction may be necessary to remove potential inhibitors of RT-LAMP? Thanks.
Jonathan Schmid-Burgk
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May 18, 2020, 5:22:42 PM5/18/20
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Hi Maya,
We are optimizing LAMP-Seq both on unpurified as well as one-step lysates of clinical samples. We will update the pre-print shortly.