Cross-contamination between samples?

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ami...@gmail.com

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Apr 11, 2020, 12:53:48 PM4/11/20
to LAMP-Seq
Amazing paper guys, I enjoyed reading your BioRxiv submission.

With a computational background, I have a rather basic question regarding cross-contamination between samples. 
Similar to performing a qPCR in a "not-so-careful-lab", it seems to be difficult to get a 'negative' measurement (i.e. a "no infection found" state), simply because the templates are floating around the lab if this is done frequently in such a scale. I was wondering with the barcoding system implemented in the LAMP-seq assay, what would happen if you get contamination of one LAMP reaction (one patient with positive infection) into another tube that is then used for a new LAMP reaction (healthy individual)?
 
Will you be able to see that this was a contamination because the sample contains the wrong barcode, or mixed barcodes? If yes, what this will look like in a sequenced dataset?

Thanks!

Jonathan Schmid-Burgk

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Apr 13, 2020, 11:56:07 AM4/13/20
to LAMP-Seq
Thanks for this thoughtful point! I would expect that contamination of an RT-LAMP reaction with the reaction product of another LAMP reaction, even when having a different barcode, will lead to false-positives that cannot be told apart by sequencing data. Therefore, it will be crucial that no LAMP reaction tube ever be opened at the site where fresh swabs are taken.
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