How handle to prepare sequencing libraries from hairpin products?

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Marco Antonio Mendoza

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Apr 26, 2020, 5:34:17 PM4/26/20
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Hi all;
actually The question is on the title...after looking at the methods on the draft manuscript, I do not understand how could we make sure that we are not losing amplified fragments during library preparation because it is on a "hairpin" configuration?
thanks for your tips.
regards
Marco Mendoza

Jonathan Schmid-Burgk

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May 18, 2020, 5:19:57 PM5/18/20
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Hi Marco,

I am not sure I understand the hairpin problem, would you mind explaining in more detail? The LAMP products are palindromic, but as they are double-stranded I don't expect them to form a hairpin. The final PCR amplicon is neither palindromic, nor contains a hairpin. Am I missing something obvious?

Thanks
Jonathan
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