Please have a look on list of primers posted on Primers of RT-LAMP thread.
Let me know your thoughts and comments
Regards
Dinesh
In the pre-print publication, we analyzed the alignment of our primers against 183 SARS-CoV-2 genomes. Every primer set perfectly matched >95% of all viral genomes. Please have a look at the supplementary file attached to the manuscript.
I think a single mismatch would inhibit the LAMP reaction but this would have to be tested experimentally.
Potentially one should use more than one set of the primers to cover all SARS-CoV-2 variants.
Thanks a lot for your comment.
Cheers,
Mikolaj
In the pre-print publication, we analyzed the alignment of our primers against 183 SARS-CoV-2 genomes. Every primer set perfectly matched >95% of all viral genomes. Please have a look at the supplementary file attached to the manuscript.
I think a single mismatch would inhibit the LAMP reaction but this would have to be tested experimentally.
Potentially one should use more than one set of the primers to cover all SARS-CoV-2 variants.