Understandin lab protocols is not my strong suite. Would you be able to describe what the single, 75 bp read structure looks like?
For (wildly fictional) example:
1-12 barcode 1
13-25 barcode 2
26-50 Orf1ab gene target
51-62 barcode 2 reverse
63-75 barcode reverse
This would give me a better sense of the analysis that needs to be done.
Thanks in advance.