Question about global frequency of positive samples

[daniel...@gmail.com]

Hi Team,

I'm not sure I understand what is meant by the sentence "as long as the global frequency of positive samples is below 1.3%".

Could you expand please?

[Jonathan Schmid-Burgk]

Hi Daniel,

For compressed barcoding, it matters that the fraction of positive samples is low, because otherwise all sub-barcodes will become positive, precluding deconvolution. Our simulations of false-positive and false-negative rates indicate that if 1.3% of samples were positive, compressed barcoding would be feasible with FPR/FNR <0.2% with the indicated parameters (10k total barcodes, 5 barcodes per sample, 3/5 detected, 10 pools per run).

Best,

Jonathan