Question about global frequency of positive samples

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daniel...@gmail.com

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Apr 25, 2020, 1:07:54 PM4/25/20
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Hi Team,
I'm not sure I understand what is meant by the sentence "as long as the global frequency of positive samples is below 1.3%".
Could you expand please?

Jonathan Schmid-Burgk

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May 18, 2020, 5:15:36 PM5/18/20
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Hi Daniel,

For compressed barcoding, it matters that the fraction of positive samples is low, because otherwise all sub-barcodes will become positive, precluding deconvolution. Our simulations of false-positive and false-negative rates indicate that if 1.3% of samples were positive, compressed barcoding would be feasible with FPR/FNR <0.2% with the indicated parameters (10k total barcodes, 5 barcodes per sample, 3/5 detected, 10 pools per run).

Best,
Jonathan
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