Re: Ghaav - The Wound Hai Full Movie 720p
Viva Allor
Directions to use: first clean wound of debris with bhaav powder (dissinfectant powder), mix Ghaav powder with distil water to create a thick paste. Apply this paste into the wound and around the area so as to pack the entire wound.
Background: Soft x-ray irradiation is usually used to treat skin-related disease in the clinic, but its effect on wound healing has not been well elucidated. The purpose of our study was to develop a wound-healing model of local soft x-ray irradiation on rats and to clarify the possible cellular mechanisms through which radiation may influence healing.
Methods: Full-thickness, 2-cm-diameter, dorsal cutaneous tissue was excised after local irradiation in rats. The dose-effect curve was recorded, and five animals irradiated with doses of 521 rad were killed at postwound days 3, 6, 9, and 15 and five were healed for histologic examination (hematoxylin and eosin staining), cell proliferation (5-bromo-2'-deoxyuridine [BrdU] incorporation), apoptosis (and terminal deoxynucleotidyl transferase biotin-dUTP nick end-labeling [TUNEL] method), and the cell cycle (flow cytometry).
Results: Soft x-ray irradiation delayed wound-healing time with an S-like curve, and the wounds that received doses in excess of 700 rad failed to heal within 40 days. This dose-effect range is far below that of gamma and hard x-ray irradiation. Relative to control wounds, which contained prominent BrdU-positive labeling at days 3 through 9 and minimal TUNEL-positive labeling during the entire healing, the irradiated wounds had less BrdU-positive labeling and significant TUNEL-positive labeling at days 3 through 9 but more BrdU-positive labeling and similar TUNEL-positive labeling at day 15. Flow cytometry studies found a higher proportion of G0/G1 phase cells (days 3-9), a lower (days 3-9) then higher (days 13-22) proportion of S phase cells, and a persistent lower proportion of G2/M phase cells (during the entire healing process) in the irradiated wounds compared with the controls.
Conclusion: Local soft x-ray irradiation could delay wound healing in a dose-dependent manner and shows a more effective biological effect than that of gamma and hard x-ray irradiation. Radiation-induced inactive cell proliferation, active cell apoptosis, and arrested cell cycle at days 3 through 9 may be one of the cellular mechanism responsible for delayed wound healing.
All animals used in this study were handled as per the international, natural, and institutional guidelines for the care and use of laboratory animals in biomedical research as promulgated by the National Research Council.7 The rats were divided into two equal groups by using a random number generator (Table 1). Animals in groups 1 and 2, after induction of soft tissue wound, were given ad libitum access to water and carbonated drink, respectively.
Section of left buccal mucosa of group 1 on post-wound day 7 showing epithelium with stratum basale (red arrow), stratum spinosum (blue arrow), and stratum granulosum (green arrow). The lamina propria contains fibroblasts (F), collagen fibers (C), blood vessels (BV), and inflammatory cells (L) forming the granulation tissue. H & E stain: photomicrograph approx. 200.
Section of left buccal mucosa of group 2 on post-wound day 7 showing epithelium with stratum basale (red arrow), stratum spinosum (blue arrow), and stratum granulosum (green arrow). The lamina propria consists of inflammatory cells (IL) and collagen fibers (C). H & E stain: photomicrograph approx. 200.
Section of left buccal mucosa of group 1d on post-wound day 21 showing stratified squamous epithelium keratinized with stratum basale (red arrow), stratum spinosum (blue arrow), stratum granulosum (green arrow), and stratum corneum (yellow arrow). The connective tissue shows collagen fiber bundles (C) and blood vessels (BV). H & E stain: photomicrograph approx. 200.
Section of left buccal mucosa of group 2d on post-wound day 21 showing acanthotic epithelium with stratum basale (red arrow), hypertrophic stratum spinosum (blue arrow), stratum granulosum (green arrow) with prominent keratohyaline granules (K), and hyperkeratotic stratum corneum (yellow arrow). Irregular rete ridges (R) are present. The lamina propria consists of fibroblasts (F), collagen bundles (C), blood vessels (BV), and inflammatory cells (IL). H & E stain: photomicrograph approx. 200.
1. The wound on his leg was deep and bleeding.
2. She suffered a head wound in the accident.
3. The knife wound on his arm required several stitches.
4. He had a small wound on his thumb from cutting vegetables.
5. The gunshot wound in his abdomen was life-threatening.
6. She had a large wound on her foot from stepping on broken glass.
7. He received a wound to his shoulder in the battle.
8. The wound on his face required plastic surgery to repair.
9. The dog bite left a nasty wound on her hand.
10. The wound on his back was infected and needed treatment.