Using Kallisto for Ribosome Profiling data

[Konstanze Simbriger]

Hello all,

I'm quite new to handling sequencing data on my own and was wondering, whether it is possible to use Kallisto for mapping ribosome profiling reads?

Generally, those reads are much shorter (28-34 nt) and most protocols/methods use adapter clipping before alignment as well as removal of ribosomal RNA from the data.

Many thanks,

Konstanze