RNA velocity (La Manno) analysis with new version (--workflow nac) of kb

[Nik Tuzov]

Hello All:

Could you give me some advice.

I am trying to reproduce this tutorial:

https://www.kallistobus.tools/tutorials/kb_velocity/python/kb_velocity/

using the new version of kb (--workflow nac).

As of Dec 14, I can't run it with kb-python 0.28.0 because kb count runs out of memory (with over 100 Gb available).

1) Is there a working version of kb-python that uses the "nac" workflow?

 
2) If not, what are the latest versions of kallisto and bustools that I can install myself and that work with "nac" worklfow?

Regards,
Nik