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p-Akt and p-eNOS utilizing particular antibodies (Cell Signaling, MA,
USA). Double-fluorescent immunohistochemical dedication of cardiomyocyte apoptosis was carried out with terminal
dUTP nick finish labelling (TUNEL) assay on deparafinized 4
μm thick sections utilizing an In Situ Cell Death Detection Equipment, fluorescein as per the package protocol (Roche Diagnostics, Mannheim, Germany).
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ABC Vectastain Elite Equipment (Vectorlabs). Reverse transcription proceeded with 1
μg of complete RNA using iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA,
USA). Regional myocardial blood movement, 14 days after
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USA) as previously described. With the use of Scion picture
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