Hi
In JASPAR we use '::' to denote TF complexes (dimers, trimers).
In this case, the Smad2::Smad3::Smad4 motif corresponds to the motif recognized by the complex, a Smad trimer. However, the motif corresponding to Smad3 looks very similar.
Could I use Smad2/3 ChIP-level antibody to detect the possible binding sites in the promoter region of interested genes using the binding motif of Smad2::Smad3::Smad4?
I would use all the Smad motifs (monomers, dimer, trimer)
Why doesn't there exist the individual TF Smad2 or Smad3 or Smad4?
Probably there is no a dedicated experiment for these TFs, so far. Other possibility is that they do not bind DNA alone.
Jaime