Document (1) discloses a process for preparing a mixed refrigerant, namely R-407C, by adding into a vessel, firstly HFC-l34a, followed by HFC-125 and finally HFC-32 (page 102, last paragraph, lines 1 to 3). The densities of HFC-134a, HFC-125 and HFC-32 are respectively 1.206, 1.190 and 0.961 (see table 1, page 5 of the patent specification). Thus, in the process disclosed in document (1), the order of introduction of the refrigerant components is such that the liquid density of the refrigerant component to be introduced afterwards is lower at the introduction temperature than the liquid density of the refrigerant component already introduced into the vessel. There is no indication in document (1) of the location of the point of introduction of the components into the vessel.
Table 1 shows the demographic and clinical characteristics of participants including sex, age, BMI, age of onset of depression, sum score of disease severity and number of episode of depression of the 96 subjects participated in the present study.
Subsequent studies have shown that serum TRP levels were decreased significantly in depression [24]. Observational studies and meta-analysis also showed that depression has an impact on serum analytes such as Vit D [7], Se [18], serotonin [24] and Mg [14]. It is also appeared from clinical data that the sum of HRDS score is greater in depressed than normal participants along with total number of episode (Table 1) that is also indicating that depression state influenced biochemical levels of various markers (Figure 1). The present study is aimed to find out the serum concentration of TRP, Vit D, Mg, Se and serotonin in depressed patients when compared to healthy volunteers. Apart from that we also correlated TRP which is a well-known biomarker in depression with other serum Vit D, Mg, Se and serotonin in depressed patients.
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