First time using JAABA for courtship experiment

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Gencay Kaan Polat

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Jul 28, 2022, 7:59:36 AM7/28/22
to JAABA
Hi everybody, 
I want to use JAABA for copulation experiment at learning but I don't know how to integrate features like recognising courting behaviours. I want to detect every movement flies do like licking, orienting, etc... My second question is I'm using mechanism that has multiple chambers for courting but I'm having problem with adjusting the background at Ctrax. I'm so grateful for your future responses.

Mayank Kabra

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Jul 28, 2022, 8:35:02 AM7/28/22
to Gencay Kaan Polat, JAABA
Hi,

I would suggest starting by detecting the individual behavior movements (like licking, orienting etc) that makeup copulation rather than using JAABA directly to detect copulation. If those are the only behaviors that are of interest, then this should suffice.

However, if you need to detect copulation then you can train a new detector where you input the predictions of these individual behavior detectors as input features. To use the predictions of previous JAABA detectors as input, after creating a JAABA project, use "Classifier -> Change Score Features..." menu to add the jab files for the individual behaviors (e.g. licking.jab, orienting.jab). And when you select features, make sure to select the "score" features.

As for detecting background in CTrax I'll suggest posting your query to the Ctrax mailing list. Or you can try using APT (https://github.com/kristinbranson/APT) with which you can track animals with more details which helps in training accurate behavior detectors in JAABA. We'll be releasing the multi-animal version of APT soon.

Mayank



On Thu, Jul 28, 2022 at 5:29 PM Gencay Kaan Polat <gencayk...@gmail.com> wrote:
Hi everybody, 
I want to use JAABA for copulation experiment at learning but I don't know how to integrate features like recognising courting behaviours. I want to detect every movement flies do like licking, orienting, etc... My second question is I'm using mechanism that has multiple chambers for courting but I'm having problem with adjusting the background at Ctrax. I'm so grateful for your future responses.

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Kristin Branson

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Jul 28, 2022, 9:05:25 AM7/28/22
to Mayank Kabra, Gencay Kaan Polat, JAABA
You could also try using the FlyTracker instead of Ctrax:
We've switched recently-ish to this software.

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