3D-CTF corrected tomograms from WARP

[Euan William Pyle]

Hi all, 

I'm trying out IsoNet2 using tomograms from WARP. These are 3D-CTF corrected, so I'm wondering about whether I should adjust some of the settings in the denoise and refine jobs. 

As the tomograms are CTF corrected, I've used the following flags in denoise and predict:

--CTF_mode None, --isCTFflipped True, --do_phaseflip_input False

Does anyone have any experience with this that can advise whether this makes sense or not?

Thanks

Euan 

[Yuntao Liu]

Hi Euan,

These are what I think should work.

 Refine or denoise for ctf phaseflipped tomos:

--CTF_mode network , --isCTFflipped True, --do_phaseflip_input (does not matter)

predict for ctf phaseflipped tomos:

--isCTFflipped True

Refine or denoise for ctf amplitude and phase corrected tomos (or disable CTF)

--CTF_mode None , --isCTFflipped True (does not matter), --do_phaseflip_input (does not matter)

predict for ctf amplitude and phase corrected tomos:

--isCTFflipped True

Yuntao Liu,  Postdoc.

California NanoSystem Institute

University of California Los Angeles

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[Euan Pyle]

Thanks Yuntao!

Best

Euan