Applying IsoNet with other filters

[Chris Lo]

Dear Dr. Liu,

As a newbie in cryo-EM, I have a conceptual question.

I would like to  compensate the missing wedge of my tomograms, with IsoNet, and denoise them with other apps such as tomo3d and topaz-denoise3D.

The filters in Tomo3d works with tiltseries during the reconstruction process. Therefore, I have to apply Tomo3D to my data before proceeding to IsoNet. My questions is, is IsoNet still functional if I train it after applying tomo3d filters? Does it make sense of treating my data with multiple software?

Thank you very much.

Best regards,

Chris

[logicvay2010]

Dear Chris,

The answer is yes, it's okay to treat your data with multiple software before Isonet.

And Isonet works better when your input tomograms have higher contrast. for example: Isonet deconv step is made for the purpose to increase contrast. 

Best,

Hui